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hydroxyurea  (MedChemExpress)


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    MedChemExpress hydroxyurea
    NF1 knockdown enhances replication stress sensitivity without altering basal proliferation in ovarian cancer cells. (a–b) Quantitative RT‐PCR showing efficient NF1 knockdown in (a) SK‐OV‐3 and (b) OVCAR‐3 cells expressing two independent NF1 shRNAs compared with control cells. (c–d) CCK‐8 assays showing comparable basal proliferation between NF1‐knockdown and control cells. (e–f) CCK‐8 assays following <t>hydroxyurea</t> treatment showing impaired growth of NF1‐deficient cells, indicating increased replication stress sensitivity. Data are presented as mean ± SEM from three independent experiments. Statistical significance was assessed using unpaired two‐tailed Student′s t ‐tests (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).
    Hydroxyurea, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydroxyurea+hy+b0313/pmc13181431-62-16-20?v=MedChemExpress
    Average 94 stars, based on 45 article reviews
    hydroxyurea - by Bioz Stars, 2026-07
    94/100 stars

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    1) Product Images from "Integrative Genomic and Functional Analysis Reveals NF1 Loss as a Modifier of DNA Damage and Replication Stress Responses in Ovarian Cancer"

    Article Title: Integrative Genomic and Functional Analysis Reveals NF1 Loss as a Modifier of DNA Damage and Replication Stress Responses in Ovarian Cancer

    Journal: Human Mutation

    doi: 10.1155/humu/9333284

    NF1 knockdown enhances replication stress sensitivity without altering basal proliferation in ovarian cancer cells. (a–b) Quantitative RT‐PCR showing efficient NF1 knockdown in (a) SK‐OV‐3 and (b) OVCAR‐3 cells expressing two independent NF1 shRNAs compared with control cells. (c–d) CCK‐8 assays showing comparable basal proliferation between NF1‐knockdown and control cells. (e–f) CCK‐8 assays following hydroxyurea treatment showing impaired growth of NF1‐deficient cells, indicating increased replication stress sensitivity. Data are presented as mean ± SEM from three independent experiments. Statistical significance was assessed using unpaired two‐tailed Student′s t ‐tests (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).
    Figure Legend Snippet: NF1 knockdown enhances replication stress sensitivity without altering basal proliferation in ovarian cancer cells. (a–b) Quantitative RT‐PCR showing efficient NF1 knockdown in (a) SK‐OV‐3 and (b) OVCAR‐3 cells expressing two independent NF1 shRNAs compared with control cells. (c–d) CCK‐8 assays showing comparable basal proliferation between NF1‐knockdown and control cells. (e–f) CCK‐8 assays following hydroxyurea treatment showing impaired growth of NF1‐deficient cells, indicating increased replication stress sensitivity. Data are presented as mean ± SEM from three independent experiments. Statistical significance was assessed using unpaired two‐tailed Student′s t ‐tests (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

    Techniques Used: Knockdown, Quantitative RT-PCR, Expressing, Control, CCK-8 Assay, Two Tailed Test



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    NF1 knockdown enhances replication stress sensitivity without altering basal proliferation in ovarian cancer cells. (a–b) Quantitative RT‐PCR showing efficient NF1 knockdown in (a) SK‐OV‐3 and (b) OVCAR‐3 cells expressing two independent NF1 shRNAs compared with control cells. (c–d) CCK‐8 assays showing comparable basal proliferation between NF1‐knockdown and control cells. (e–f) CCK‐8 assays following hydroxyurea treatment showing impaired growth of NF1‐deficient cells, indicating increased replication stress sensitivity. Data are presented as mean ± SEM from three independent experiments. Statistical significance was assessed using unpaired two‐tailed Student′s t ‐tests (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

    Journal: Human Mutation

    Article Title: Integrative Genomic and Functional Analysis Reveals NF1 Loss as a Modifier of DNA Damage and Replication Stress Responses in Ovarian Cancer

    doi: 10.1155/humu/9333284

    Figure Lengend Snippet: NF1 knockdown enhances replication stress sensitivity without altering basal proliferation in ovarian cancer cells. (a–b) Quantitative RT‐PCR showing efficient NF1 knockdown in (a) SK‐OV‐3 and (b) OVCAR‐3 cells expressing two independent NF1 shRNAs compared with control cells. (c–d) CCK‐8 assays showing comparable basal proliferation between NF1‐knockdown and control cells. (e–f) CCK‐8 assays following hydroxyurea treatment showing impaired growth of NF1‐deficient cells, indicating increased replication stress sensitivity. Data are presented as mean ± SEM from three independent experiments. Statistical significance was assessed using unpaired two‐tailed Student′s t ‐tests (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

    Article Snippet: To assess the effect of NF1 knockdown on sensitivity to replication stress, cells were treated with hydroxyurea (HU, 0.25 mM, MCE) 24 h after seeding.

    Techniques: Knockdown, Quantitative RT-PCR, Expressing, Control, CCK-8 Assay, Two Tailed Test